Seahorse XF-24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format, Seahorse-XFe96 – in 96-well format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells. The description of the systems can be found: https://www.agilent.com/en/product/cell-analysis/real-time-cell-metabolic-analysis/xf-analyzers 


  • Real-time Results- this integrated system reports metabolic rates in just minutes, without sample extraction or labeling.  Wave software controls the instrument and performs rate measurements on the fly for same day results.
  • Live-Cell Responses – detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing while maintaining physiologic temperature (37oC).
  • Sensitive- analyze as few as 10,000 cells per well in the custom 24-well plate.  Cell number requirements vary by cell type; consult the Cell Reference Database for details. The 24-well microplate and system has capacity for larger and/or more metabolically active samples than the 96-well system. The 96-well system requires smaller cell number, and can accommodate more samples simultaneously.
  • Compatible with a variety of samples due to the precision-controlled heating tray, which can maintain 16-42oC (12oC-20oC above ambient conditions).
  • Analyze function of islet or other mobile samples with the Seahorse XF24 Islet Capture Plate
  • Measure mitochondrial function with the Seahorse XF Cell Mito Stress Test
  • Generate a metabolic phenotype within one hour with the Seahorse XF Cell Energy Phenotype Test
  • Measure glycolytic rates in live cells with the Seahorse XF Glycolytic Rate Assay
  • Quickly determine dependency of cellular energy production on mitochondrial substrates with the Seahorse XF Mito Fuel Flex Test
  • Easily create assay protocols and analyze data.
  • For Research Use Only. Not for use in diagnostic procedures.

Seahorse Instruments

ItemBrandCatalog Number
Seahorse XF24 Flux PackAgilent Technologies100850-001*
Seahorse XFe96 Flux PackAgilent Technologies102416-100
Palmitoyl-L-carnitine chlorideSigmaP1645
Sodium PalmitateSigma
P9767
MalonateSigma
M1875
(+/-) Sodium 3 hydroxybutyrateSigma
54965
PyruvateSigma
S8636
GlutamateSigma
G8415
MalateSigma
M6413
SuccinateSigma
S2378
ADPSigma
A2754, A5285
RotenoneSigma
R8875
OligomycinSigma
O4876
FCCPSigma
C2920
Antimycin ASigma
A8674
GlucoseSigma
G8270
2-deoxyglucoseSigma
D6134
CarnitineSigma
C0158
XF DMEM Medium, pH 74Agilent Technologies103575-100


Seahorse

Users are responsible for purchasing the supplies and reagents, some of the reagents are listed in the table below

Seahorse

Seahorse XF24 and XFe96 Analyzers

Equipment Description and Usage Service

* The XF24 Flux packs are no longer available; therefore the XF24 system will be supported until users will run out of their XF24 supplies.

UC customers: $229/assay
DARTs/NonProfits: $306/assay
External/For Profit: $351/assay


For questions or to get started, please contact unit director Alexey Tomilov, PhD, at atomilov@ucdavis.edu.
Or call 530-902-1051

The Seahorse XF flux analyzer equipment is available for recharge use at the UC Davis. The 24-well Seahorse system provides researchers the opportunity to perform medium-throughput, real-time measurement of mitochondrial function and cellular bioenergetics in an expedited manner compared to traditional techniques. Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis. XF Analyzers perform compound addition and mixing, label-free analytical detection, and automatic measurement of OCR and ECAR in real time (https://www.agilent.com/en/products/cell-analysis/seahorse-analyzers).

UC customers: $229/assay
DARTs/NonProfits: $306/assay
External/For Profit: $351/assay


For questions or to get started, please contact unit director Alexey Tomilov, PhD, at atomilov@ucdavis.edu.
Or call 530-902-1051

UC customers: $229/assay
DARTs/NonProfits: $306/assay
External/For Profit: $351/assay


For questions or to get started, please contact unit director Alexey Tomilov, PhD, at atomilov@ucdavis.edu.
Or call 530-902-1051